|
|
|
|
|
| Benslimane-ahmim, Z.; Heymann, D.; Dizier, B.; Lokajczyk, A.; Brion, R.; Laurendeau, I.; Bieche, I.; Smadja, D. M.; Galy-fauroux, I.; Colliec-jouault, Sylvia; Fischer, Anne-marie; Boisson-vidal, Catherine. |
| Background: Osteoprotegerin (OPG), a soluble receptor of the tumour necrosis factor family, and its ligand, the receptor activator of nuclear factor-kappa B ligand (RANKL), are emerging as important regulators of vascular pathophysiology. Objectives: We evaluated their effects on vasculogenesis induced by endothelial colony-forming cells (ECFC) and on neovessel formation in vivo. Methods: Effects of OPG and RANKL on in vitro angiogenesis were evaluated after ECFC incubation with OPG or RANKL (0-50 ng mL(-1)). Effects on microvessel formation were evaluated with an in vivo murin Matrigel plug assay. Vascularization was evaluated by measuring plug hemoglobin and vascular endothelial growth factor (VEGF)-R2 content 14 days after implantation. Results: We... |
| Tipo: Text |
Palavras-chave: Endothelial colony-forming cells; Osteoprotegerin; Receptor activator of nuclear factor-kappa B ligand; Vasculogenesis. |
| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00033/14462/11863.pdf |
| |
|
|
| Sarlon, G.; Zemani, F.; David, L.; Van Huyen, J. -p. Duong; Dizier, B.; Grelac, F.; Colliec-jouault, Sylvia; Galy-fauroux, Isabelle; Bruneval, P.; Fischer, Anne-marie; Emmerich, J.; Boisson-vidal, Catherine. |
| . Background: Fucoidan, an antithrombotic polysaccharide, can induce endothelial colony-forming cells (ECFC) to adopt an angiogenic phenotype in vitro. Objectives: We evaluated the effect of fucoidan on vasculogenesis induced by ECFC in vivo. Methods: We used a murine hindlimb ischemia model to probe the synergic role of fucoidan-treatment and ECFC infusion during tissue repair. Results: We found that exposure of ECFC to fucoidan prior to their intravenous injection improved residual muscle blood flow and increased collateral vessel formation. Necrosis of ischemic tissue was significantly reduced on day 14, to 12.1% of the gastronecmius cross-sectional surface area compared with 40.1% in animals injected with untreated-ECFC. ECFC stimulation with fucoidan... |
| Tipo: Text |
Palavras-chave: Angiogenesis; Endothelial colony-forming cells; Fucoidan; Ischemia; Revascularization. |
| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00065/17616/18204.pdf |
| |
|
|
|
